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weight genomic dna  (Thermo Fisher)


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    Thermo Fisher weight genomic dna
    Weight Genomic Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/weight+genomic+dna/DNA/pm41320126-68-0-17
    Average 99 stars, based on 1 article reviews
    weight genomic dna - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Role of the rs6184 growth hormone receptor gene polymorphism in mandibular morphogenesis.
    Article Snippet: Background: Polymorphisms in the growth hormone receptor (GHR) gene influence the craniofacial system by affecting the growth of the mandibular body length and ramal height, with significant variations observed across different ethnic populations.. This study investigated the relationship between a single nucleotide polymorphism (rs6184) in the GHR gene and its effects on mandibular morphogenesis in a Dravidian population.. Methods: A total of 250 subjects were divided into three groups.

    Isolation:

    Article Title: Role of the rs6184 growth hormone receptor gene polymorphism in mandibular morphogenesis.
    Article Snippet: Background: Polymorphisms in the growth hormone receptor (GHR) gene influence the craniofacial system by affecting the growth of the mandibular body length and ramal height, with significant variations observed across different ethnic populations.. This study investigated the relationship between a single nucleotide polymorphism (rs6184) in the GHR gene and its effects on mandibular morphogenesis in a Dravidian population.. Methods: A total of 250 subjects were divided into three groups.

    Article Title: Chromosome-level genome assembly of Cylas formicarius provides insights into its adaptation and invasion mechanisms
    Article Snippet: .. High-molecular-weight genomic DNA was isolated from approximately 400 female weevils using a TreliefTM Animal Genomic DNA Kit (Tsingke Biological Technology, China), and the DNA quality and quantity were assessed using a NanoDropTM spectrophotometer (Thermo Fisher Scientific, USA) and a Qubit® 3.0 Fluorometer (Invitrogen, USA). .. The extracted DNA was then used to construct Illumina libraries and PacBio RSII libraries.

    Article Title: Building a foundation for gene family analysis in Rosaceae genomes with a novel workflow: A case study in Pyrus architecture genes
    Article Snippet: .. A CTAB isolation protocol ( ) was used to generate high-molecular-weight genomic DNA with the following modifications: the extraction was performed at large-scale with 100 ml of extraction buffer in a 250 ml Nalgene centrifuge bottle; the isopropanol precipitation was performed at room temperature (~ 5 minutes) followed immediately by centrifugation; after a 15-minute incubation in the first pellet wash solution, the pellet was transferred to a 50 ml centrifugation tube via sterile glass hook before performing the second pellet wash; following the second pellet wash, centrifugation, and air drying, the pellet was resuspended in 2 ml TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) and allowed to resuspend at 4°C overnight. .. The concentration of the DNA was measured by a Qubit 2.0 fluorometer (Invitrogen) and 50 ug DNA was digested with RNase A (Qiagen, final concentration 10 ug/ml, 37°C for 30 minutes) and then further cleaned up using the PacBio recommended, user-shared gDNA clean-up protocol ( https://www.pacb.com/search/?q=user+shared+protocols ) performed at large-scale with the DNA sample brought up to 2 ml with TE and all other volumes scaled up accordingly.

    Gentle:

    Article Title: Optical Genome Mapping versus Whole-Genome Sequencing in the Clinical Diagnosis of Gynecologic Mesenchymal Tumors.
    Article Snippet: .. High-molecular-weight genomic DNA was extracted using Nanobind magnetic disks and subjected to gentle mixing on a HulaMixer (Thermo Fisher Scientific, Waltham, MA). .. DNA concentration was quantified with the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific).

    Agarose Gel Electrophoresis:

    Article Title: Malignant clonal evolution drives multiple myeloma cellular ecological diversity and microenvironment reprogramming.
    Article Snippet: The sequencing procedure was performed according to standard Oxford Nanopore Technologies protocols [20]. .. First, high-molecular-weight genomic DNA was extracted from the BM aspirate using HiPure Tissue & Blood DNA Kit (D3018–03, Magen), and Nanodrop, Qubit, and 0.35% agarose gel electrophoresis were used for purity, concentration, and integrity quality inspection. ..

    Article Title: Malignant clonal evolution drives multiple myeloma cellular ecological diversity and microenvironment reprogramming
    Article Snippet: The sequencing procedure was performed according to standard Oxford Nanopore Technologies protocols [ ]. .. First, high-molecular-weight genomic DNA was extracted from the BM aspirate using HiPure Tissue & Blood DNA Kit (D3018–03, Magen), and Nanodrop, Qubit, and 0.35% agarose gel electrophoresis were used for purity, concentration, and integrity quality inspection. ..

    Concentration Assay:

    Article Title: Malignant clonal evolution drives multiple myeloma cellular ecological diversity and microenvironment reprogramming.
    Article Snippet: The sequencing procedure was performed according to standard Oxford Nanopore Technologies protocols [20]. .. First, high-molecular-weight genomic DNA was extracted from the BM aspirate using HiPure Tissue & Blood DNA Kit (D3018–03, Magen), and Nanodrop, Qubit, and 0.35% agarose gel electrophoresis were used for purity, concentration, and integrity quality inspection. ..

    Article Title: Malignant clonal evolution drives multiple myeloma cellular ecological diversity and microenvironment reprogramming
    Article Snippet: The sequencing procedure was performed according to standard Oxford Nanopore Technologies protocols [ ]. .. First, high-molecular-weight genomic DNA was extracted from the BM aspirate using HiPure Tissue & Blood DNA Kit (D3018–03, Magen), and Nanodrop, Qubit, and 0.35% agarose gel electrophoresis were used for purity, concentration, and integrity quality inspection. ..

    Article Title: Multiplex Editing of the Nucleoredoxin1 Tandem Array in Poplar: From Small Indels to Translocations and Complex Inversions
    Article Snippet: .. High-molecular-weight genomic DNA was extracted from young leaves of greenhouse-grown plants using the cetyltrimethylammonium bromide method, and concentration determined by Qubit dsDNA HS Assay Kit (Invitrogen, Waltham, MA). .. For each of the samples, 700 ng genomic DNA in 55 μL ddH 2 O was sheared to ∼550 bp using Covaris E220 (Woburn, MA) and size-selected using AMPure XP beads (Beckman Coulter, Indianapolis, IN).

    Spectrophotometry:

    Article Title: Chromosome-level genome assembly of Cylas formicarius provides insights into its adaptation and invasion mechanisms
    Article Snippet: .. High-molecular-weight genomic DNA was isolated from approximately 400 female weevils using a TreliefTM Animal Genomic DNA Kit (Tsingke Biological Technology, China), and the DNA quality and quantity were assessed using a NanoDropTM spectrophotometer (Thermo Fisher Scientific, USA) and a Qubit® 3.0 Fluorometer (Invitrogen, USA). .. The extracted DNA was then used to construct Illumina libraries and PacBio RSII libraries.

    Purification:

    Article Title: Deep cryptic diversity in the Craugastor podiciferus Species Group (Anura: Craugastoridae) of Isthmian Central America revealed by mitochondrial and nuclear data
    Article Snippet: .. High-molecular-weight genomic DNA was further purified with RNase A, examined for quality on agarose gels, and quantified with a Qubit 2.0 fluorometer (Thermo Fisher Scientific). ..

    Extraction:

    Article Title: Building a foundation for gene family analysis in Rosaceae genomes with a novel workflow: A case study in Pyrus architecture genes
    Article Snippet: .. A CTAB isolation protocol ( ) was used to generate high-molecular-weight genomic DNA with the following modifications: the extraction was performed at large-scale with 100 ml of extraction buffer in a 250 ml Nalgene centrifuge bottle; the isopropanol precipitation was performed at room temperature (~ 5 minutes) followed immediately by centrifugation; after a 15-minute incubation in the first pellet wash solution, the pellet was transferred to a 50 ml centrifugation tube via sterile glass hook before performing the second pellet wash; following the second pellet wash, centrifugation, and air drying, the pellet was resuspended in 2 ml TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) and allowed to resuspend at 4°C overnight. .. The concentration of the DNA was measured by a Qubit 2.0 fluorometer (Invitrogen) and 50 ug DNA was digested with RNase A (Qiagen, final concentration 10 ug/ml, 37°C for 30 minutes) and then further cleaned up using the PacBio recommended, user-shared gDNA clean-up protocol ( https://www.pacb.com/search/?q=user+shared+protocols ) performed at large-scale with the DNA sample brought up to 2 ml with TE and all other volumes scaled up accordingly.

    Centrifugation:

    Article Title: Building a foundation for gene family analysis in Rosaceae genomes with a novel workflow: A case study in Pyrus architecture genes
    Article Snippet: .. A CTAB isolation protocol ( ) was used to generate high-molecular-weight genomic DNA with the following modifications: the extraction was performed at large-scale with 100 ml of extraction buffer in a 250 ml Nalgene centrifuge bottle; the isopropanol precipitation was performed at room temperature (~ 5 minutes) followed immediately by centrifugation; after a 15-minute incubation in the first pellet wash solution, the pellet was transferred to a 50 ml centrifugation tube via sterile glass hook before performing the second pellet wash; following the second pellet wash, centrifugation, and air drying, the pellet was resuspended in 2 ml TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) and allowed to resuspend at 4°C overnight. .. The concentration of the DNA was measured by a Qubit 2.0 fluorometer (Invitrogen) and 50 ug DNA was digested with RNase A (Qiagen, final concentration 10 ug/ml, 37°C for 30 minutes) and then further cleaned up using the PacBio recommended, user-shared gDNA clean-up protocol ( https://www.pacb.com/search/?q=user+shared+protocols ) performed at large-scale with the DNA sample brought up to 2 ml with TE and all other volumes scaled up accordingly.

    Incubation:

    Article Title: Building a foundation for gene family analysis in Rosaceae genomes with a novel workflow: A case study in Pyrus architecture genes
    Article Snippet: .. A CTAB isolation protocol ( ) was used to generate high-molecular-weight genomic DNA with the following modifications: the extraction was performed at large-scale with 100 ml of extraction buffer in a 250 ml Nalgene centrifuge bottle; the isopropanol precipitation was performed at room temperature (~ 5 minutes) followed immediately by centrifugation; after a 15-minute incubation in the first pellet wash solution, the pellet was transferred to a 50 ml centrifugation tube via sterile glass hook before performing the second pellet wash; following the second pellet wash, centrifugation, and air drying, the pellet was resuspended in 2 ml TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) and allowed to resuspend at 4°C overnight. .. The concentration of the DNA was measured by a Qubit 2.0 fluorometer (Invitrogen) and 50 ug DNA was digested with RNase A (Qiagen, final concentration 10 ug/ml, 37°C for 30 minutes) and then further cleaned up using the PacBio recommended, user-shared gDNA clean-up protocol ( https://www.pacb.com/search/?q=user+shared+protocols ) performed at large-scale with the DNA sample brought up to 2 ml with TE and all other volumes scaled up accordingly.

    Sterility:

    Article Title: Building a foundation for gene family analysis in Rosaceae genomes with a novel workflow: A case study in Pyrus architecture genes
    Article Snippet: .. A CTAB isolation protocol ( ) was used to generate high-molecular-weight genomic DNA with the following modifications: the extraction was performed at large-scale with 100 ml of extraction buffer in a 250 ml Nalgene centrifuge bottle; the isopropanol precipitation was performed at room temperature (~ 5 minutes) followed immediately by centrifugation; after a 15-minute incubation in the first pellet wash solution, the pellet was transferred to a 50 ml centrifugation tube via sterile glass hook before performing the second pellet wash; following the second pellet wash, centrifugation, and air drying, the pellet was resuspended in 2 ml TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) and allowed to resuspend at 4°C overnight. .. The concentration of the DNA was measured by a Qubit 2.0 fluorometer (Invitrogen) and 50 ug DNA was digested with RNase A (Qiagen, final concentration 10 ug/ml, 37°C for 30 minutes) and then further cleaned up using the PacBio recommended, user-shared gDNA clean-up protocol ( https://www.pacb.com/search/?q=user+shared+protocols ) performed at large-scale with the DNA sample brought up to 2 ml with TE and all other volumes scaled up accordingly.



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